OPN (encoded by SPP1 and OPN) was purchased from Shanghai Hengyuan Biochemical Reagent Co., Ltd. (Shanghai, China). LPS (HY-D1056) and recombinant OPN (HY-P78358) derived from mice were acquired from MedChemExpress (Monmouth Junction, NJ, USA). Rabbit OPN, ??-SMA, CD68, Col I, Col III, and ??-actin primary antibodies were sourced from Cell Signaling Technology (Danvers, MA, USA).